National Diagnostics HISTO-CLEAR II 1 GL

• Djoumana
• November 26, 2021
• 0

Histo-Clear II

• Low Hazard
  
• Biodegradable
  
• Lowered Citrus Odor

Primarily probably the most usually used histological stain, hematoxylin is a pure compound extracted from a species of tree current in Mexico and the West Indies. The extracted compound is oxidized to supply hematein, the energetic staining ingredient of the hematoxylin stain.
The manufacturing of histological staining choices is an art work. Just some laboratories are in a position to making a stain of reliable vibrance. At Nationwide Diagnostics we’ve now gone one step further. Our proprietary methods acquire the most effective administration of the ripening course of, resulting in the one commercially on the market Harris’ Hematoxylin with a full one 12 months shelf life.

 

Histo-Clear II, like its predecessor Histo-Clear, is a safer histological clearing agent that leads to the manufacturing of high-quality tissue slides. Histo-Clear II is unhazardous and totally biodegradable, thus decreasing disposal costs. Histo-Clear II has a vastly diminished citrus odor as compared with totally different citrus-based histological clearing brokers.

Discover: Histo-Clear II is simply not appropriate with many synthetic mounting media. Histo-Clear II is a long-standing favorite dry mounting procedures and aqueous mounting. Please affirm compatibility alongside along with your mounting media sooner than using Histo-Clear II with in wet-mounting with synthetic media.

 

Hazardous Provides Cost (HazMat) applies if shipped by Air.

PROCEDURE
Discover: The following protocol has given good to fantastic outcomes with all types of tissue sections. It
may ought to be optimized for a given tissue, half
thickness, and so forth.

 

 

1. Dewax and rehydrate slides

a) Immerse slides inside the following sequence of baths:
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
l 100% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 50% ethanol – 2 minutes
l Water – 2 minutes

2. Stain
a) Immerse slides in Hematoxylin reply for 10- 20 minutes.
b) Rinse 2 minutes in water or Scott’s Tapwater substitute.
c) Differentiate with 2-5 dips in 70% ethanol containing 0.1% HCl.
d) Immerse for 2 minutes in Scott’s Tapwater substitute.
e) Stain in 1% aqueous Eosin with 0.1 acetic acid for 10 minutes.

3. Dehydrate, clear and mount
a) Immerse slides inside the following sequence of
baths:
l 50% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 100% ethanol – 2 minutes
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
b) Mount coverslip with Histomount.

 

10X PBS MaxTag Histo (OKRA00043)
OKRA00043	Aviva Systems Biology	100 ml	EUR 168
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Hematoxylin Counterstain MaxTag Histo (OKRA00050)
OKRA00050	Aviva Systems Biology	15 ml	EUR 168
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

25ML Formamide CE
NAT1096	National Diagnostics	25ML	Ask for price

4L ScintLogic U
NAT1532	National Diagnostics	4L	EUR 220.8

20L FlowLogic U
NAT1534	National Diagnostics	20L	EUR 907.2

Test product
testtt	National Diagnostics	0	Ask for price

Streptavidin-Peroxidase Conjugate MaxTag Histo (OKRA00036)
OKRA00036	Aviva Systems Biology	1 each	EUR 339.6
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

PolyMount Mounting Media MaxTag Histo (OKRA00045)
OKRA00045	Aviva Systems Biology	15 ml	EUR 151.2
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

384 Clear Wells Clear Frame
PCR0430	Scientific Laboratory Supplies	PK50	EUR 248.52

Biotin anti-IgG Antibody MaxTag Histo (OKRA00041)
OKRA00041	Aviva Systems Biology	1 each	EUR 339.6
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Calci-Clear
NAT1312	Scientific Laboratory Supplies	EACH	EUR 38.08

Calci-Clear
NAT1314	Scientific Laboratory Supplies	EACH	EUR 107.16

Calci-Clear
NAT1316	Scientific Laboratory Supplies	EACH	EUR 451.44

Centrifuge Tube
C1005-T5*-Clear	Benchmark Scientific	1 PC	EUR 117.16

Histo-Blood Group ABO System Transferase (ABO) Antibody
20-abx141615	Abbexa	EUR 444.00 EUR 727.20 EUR 360.00	100 ul 200 ul 50 ul

Histo-Blood Group ABO System Transferase (ABO) Antibody
abx117115-100ug	Abbexa	100 ug	EUR 560.4

Histo-Blood Group ABO System Transferase (ABO) Antibody
20-abx001336	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

Histo-Blood Group ABO System Transferase (ABO) Antibody
abx029931-400ul	Abbexa	400 ul	EUR 627.6

Histo-Blood Group ABO System Transferase (ABO) Antibody
abx029931-80l	Abbexa	80 µl	EUR 343.2

Histo-Blood Group ABO System Transferase (ABO) Antibody
20-abx339880	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Histo-Blood Group ABO System Transferase (ABO) Antibody
20-abx339881	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Histo-Blood Group ABO System Transferase (ABO) Antibody
20-abx315565	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Histo-Blood Group ABO System Transferase (ABO) Antibody
abx215750-100ug	Abbexa	100 ug	EUR 526.8

Biotin anti-Rabbit IgG Antibody MaxTag Histo (OKRA00042)
OKRA00042	Aviva Systems Biology	1 each	EUR 339.6
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Biotin anti-Human IgG Antibody MaxTag Histo (OKRA00047)
OKRA00047	Aviva Systems Biology	1 each	EUR 339.6
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

EZ-10 RNA Column and collection tube (clear tube, clear ring, clear collection)
SD5008	Bio Basic	100, 100UNIT	EUR 91.06

384 WELL CLEAR RIGI PLATE PCR MICROPLATE. CLEAR
PCR-384-RGD-C	CORNING	20/pk	EUR 550.8
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Human Kidney Slide (Clear Cell Renal Cell Carcinoma) (5 slides/pk)
HTS-10405	Alpha Diagnostics	1 pk	EUR 343.2

0.6ML MICROTUBES. CLEAR
MCT-060-C	CORNING	1000/pk	EUR 212.4
Description: Micro Tubes; Micro Tubes Push Cap- Axygen

1.7ML MICROTUBES. CLEAR
MCT-175-C	CORNING	500/pk	EUR 130.8
Description: Micro Tubes; Micro Tubes Push Cap- Axygen

1000UL CLEAR TIPS
T-1000-C	CORNING	1000/pk	EUR 97.2
Description: Non Filter Tips; Pipette Tips - Axygen