• November 26, 2021
quee bio Human baff r

qayee bio human baff r

The ELISA gear relies on commonplace sandwich enzyme-linked immune-sorbent assay expertise. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Requirements (Expression system for common: NSO; Immunogen sequence: S7-A71) and try samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer.

Avidin-Biotin-Peroxidase Subtle is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualise HRP enzymatic response. TMB is catalyzed by HRP to supply a blue shade product that turned yellow after along with acidic cease reply. The density of yellow is proportional to the Human BAFF-R quantity of pattern captured in plate.

Key decisions and particulars

  • Sensitivity: 10 pg/ml
  • Differ: 78 pg/ml – 5000 pg/ml
  • Pattern kind: Cell customized supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay kind: Sandwich (quantitative)
  • Reacts with: Human
  • Product set up

    Human BAFF-R ELISA Instruments
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Pattern n Point out SD CV%
    1 16 147pg/ml 10.29 = 7%
    2 16 683pg/ml 49.85 = 7.3%
    3 16 2750pg/ml 206.25 = 7.5%
    Inter-assay
    Pattern n Point out SD CV%
    1 24 156pg/ml 13.88 = 8.9%
    2 24 700pg/ml 62.3 = 8.9%
    3 24 2531pg/ml 227.05 = 8.9%
  • Pattern kind

    Cell customized supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
  • Assay kind

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Differ

    78 pg/ml – 5000 pg/ml
  • Assay time

    3h 30m
  • Assay dimension

    Loads of steps commonplace assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell customized supernatants, cell lysates, serum and plasma (heparin, EDTA).

    bio human baff r
    bio human baff r
  • Notes

    BAFF-R (moreover generally known as Tumor necrosis problem receptor superfamily member 13C or B-cell-activating problem receptor)  is a protein that in people is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating problem (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich area. It’s thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

  • Platform

    Pre-coated microplate (12 x Eight appropriately strips)

Histone Acetyl H4ac Peptide, Biotinylated

R-1008
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H3K9ac Peptide

R-1009
  • EUR 267.06
  • EUR 78.10
  • EUR 140.80
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H3K9ac Peptide, Biotinylated

R-1010
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H3K14ac Peptide

R-1011
  • EUR 267.06
  • EUR 78.10
  • EUR 140.80
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H3K14ac Peptide, Biotinylated

R-1012
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H4K5ac Peptide

R-1014
  • EUR 267.06
  • EUR 78.10
  • EUR 140.80
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H4K8ac Peptide

R-1015
  • EUR 267.06
  • EUR 78.10
  • EUR 140.80
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H4K12ac Peptide

R-1016
  • EUR 267.06
  • EUR 78.10
  • EUR 140.80
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H4K16ac Peptide

R-1017
  • EUR 267.06
  • EUR 78.10
  • EUR 140.80
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K4me1 Monomethyl Peptide

R-1018
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K4me1 Monomethyl Peptide, Biotinylated

R-1019
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K4me2 Dimethyl Peptide

R-1020
  • EUR 267.06
  • EUR 78.10
  • EUR 140.80
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K4me2 Dimethyl Peptide, Biotinylated

R-1021
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K4me3 Trimethyl Peptide

R-1022
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K4me3 Trimethyl Peptide, Biotinylated

R-1023
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me1 Monomethyl Peptide

R-1024
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me1 Monomethyl Peptide, Biotinylated

R-1025
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me2 Dimethyl Peptide

R-1026
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me2 Dimethyl Peptide, Biotinylated

R-1027
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me3 Trimethyl Peptide

R-1028
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me3 Trimethyl Peptide, Biotinylated

R-1029
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me1 Monomethyl Peptide

R-1030
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me1 Monomethyl Peptide, Biotinylated

R-1031
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me2 Dimethyl Peptide

R-1032
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me2 Dimethyl Peptide, Biotinylated

R-1033
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me3 Trimethyl Peptide

R-1034
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me3 Trimethyl Peptide, Biotinylated

R-1035
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K36me1 Monomethyl Peptide

R-1036
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K36me1 Monomethyl Peptide, Biotinylated

R-1037
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

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