Genetic Disorders in Arabs

qayee bio human baff r

• Djoumana
• November 26, 2021
• 0

The ELISA gear relies on commonplace sandwich enzyme-linked immune-sorbent assay expertise. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Requirements (Expression system for common: NSO; Immunogen sequence: S7-A71) and try samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer.

Avidin-Biotin-Peroxidase Subtle is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualise HRP enzymatic response. TMB is catalyzed by HRP to supply a blue shade product that turned yellow after along with acidic cease reply. The density of yellow is proportional to the Human BAFF-R quantity of pattern captured in plate.

Key decisions and particulars

• Sensitivity: 10 pg/ml
• Differ: 78 pg/ml – 5000 pg/ml
• Pattern kind: Cell customized supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
• Detection method: Colorimetric
• Assay kind: Sandwich (quantitative)
• Reacts with: Human

• Product set up

  Human BAFF-R ELISA Instruments

• Detection method

  Colorimetric

• Precision

  Intra-assay

  Pattern	n	Point out	SD	CV%
  1	16	147pg/ml	10.29	= 7%
  2	16	683pg/ml	49.85	= 7.3%
  3	16	2750pg/ml	206.25	= 7.5%

  Inter-assay

  Pattern	n	Point out	SD	CV%
  1	24	156pg/ml	13.88	= 8.9%
  2	24	700pg/ml	62.3	= 8.9%
  3	24	2531pg/ml	227.05	= 8.9%

• Pattern kind

  Cell customized supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma

• Assay kind

  Sandwich (quantitative)

• Sensitivity

  < 10 pg/ml

• Differ

  78 pg/ml – 5000 pg/ml

• Assay time

  3h 30m

• Assay dimension

  Loads of steps commonplace assay

• Species reactivity

  Reacts with: Human

• Product overview

  The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell customized supernatants, cell lysates, serum and plasma (heparin, EDTA).

  

• Notes

  BAFF-R (moreover generally known as Tumor necrosis problem receptor superfamily member 13C or B-cell-activating problem receptor)  is a protein that in people is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating problem (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich area. It’s thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

• Platform

  Pre-coated microplate (12 x Eight appropriately strips)

Histone Acetyl H4ac Peptide, Biotinylated
R-1008	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone Acetyl H3K9ac Peptide
R-1009	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H3K9ac Peptide, Biotinylated
R-1010	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone Acetyl H3K14ac Peptide
R-1011	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H3K14ac Peptide, Biotinylated
R-1012	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone Acetyl H4K5ac Peptide
R-1014	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H4K8ac Peptide
R-1015	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H4K12ac Peptide
R-1016	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H4K16ac Peptide
R-1017	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone H3K4me1 Monomethyl Peptide
R-1018	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K4me1 Monomethyl Peptide, Biotinylated
R-1019	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K4me2 Dimethyl Peptide
R-1020	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone H3K4me2 Dimethyl Peptide, Biotinylated
R-1021	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K4me3 Trimethyl Peptide
R-1022	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K4me3 Trimethyl Peptide, Biotinylated
R-1023	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K9me1 Monomethyl Peptide
R-1024	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K9me1 Monomethyl Peptide, Biotinylated
R-1025	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K9me2 Dimethyl Peptide
R-1026	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K9me2 Dimethyl Peptide, Biotinylated
R-1027	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K9me3 Trimethyl Peptide
R-1028	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K9me3 Trimethyl Peptide, Biotinylated
R-1029	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K27me1 Monomethyl Peptide
R-1030	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K27me1 Monomethyl Peptide, Biotinylated
R-1031	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K27me2 Dimethyl Peptide
R-1032	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K27me2 Dimethyl Peptide, Biotinylated
R-1033	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K27me3 Trimethyl Peptide
R-1034	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K27me3 Trimethyl Peptide, Biotinylated
R-1035	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K36me1 Monomethyl Peptide
R-1036	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K36me1 Monomethyl Peptide, Biotinylated
R-1037	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul