• November 26, 2021
quee bio Human baff r

qayee bio human baff r

The ELISA gear relies on commonplace sandwich enzyme-linked immune-sorbent assay expertise. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Requirements (Expression system for common: NSO; Immunogen sequence: S7-A71) and try samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer.

Avidin-Biotin-Peroxidase Subtle is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualise HRP enzymatic response. TMB is catalyzed by HRP to supply a blue shade product that turned yellow after along with acidic cease reply. The density of yellow is proportional to the Human BAFF-R quantity of pattern captured in plate.

Key decisions and particulars

  • Sensitivity: 10 pg/ml
  • Differ: 78 pg/ml – 5000 pg/ml
  • Pattern kind: Cell customized supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay kind: Sandwich (quantitative)
  • Reacts with: Human
  • Product set up

    Human BAFF-R ELISA Instruments
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Pattern n Point out SD CV%
    1 16 147pg/ml 10.29 = 7%
    2 16 683pg/ml 49.85 = 7.3%
    3 16 2750pg/ml 206.25 = 7.5%
    Inter-assay
    Pattern n Point out SD CV%
    1 24 156pg/ml 13.88 = 8.9%
    2 24 700pg/ml 62.3 = 8.9%
    3 24 2531pg/ml 227.05 = 8.9%
  • Pattern kind

    Cell customized supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
  • Assay kind

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Differ

    78 pg/ml – 5000 pg/ml
  • Assay time

    3h 30m
  • Assay dimension

    Loads of steps commonplace assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell customized supernatants, cell lysates, serum and plasma (heparin, EDTA).

    bio human baff r
    bio human baff r
  • Notes

    BAFF-R (moreover generally known as Tumor necrosis problem receptor superfamily member 13C or B-cell-activating problem receptor)  is a protein that in people is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating problem (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich area. It’s thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

  • Platform

    Pre-coated microplate (12 x Eight appropriately strips)

Polyclonal BAFF-R Antibody

APR15125G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BAFF-R . This antibody is tested and proven to work in the following applications:

BAFF-R Polyclonal Antibody

3578-100
EUR 316

BAFF-R Blocking Peptide

3578RBP-50
EUR 153

BAFF-R Recombinant Protein

90-237 50 ug
EUR 500.75
Description: BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation . Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases, such as Sjögren’s syndrome , Rheumatoid Arthritis (RA) , Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE) . BAFF is also increased levels in some lymphoid cancers.

Anti-BAFF-R (human) Monoclonal Antibody (11C1)

M02865 100ug
EUR 446
Description: Mouse Monoclonal BAFF-R (human) Antibody (11C1). Validated in Flow Cytometry, IHC and tested in Human.

Human B-Cell Activating Factor (BAFF) ELISA Kit

RD-BAFF-Hu-48Tests 48 Tests
EUR 500

Human B-Cell Activating Factor (BAFF) ELISA Kit

RD-BAFF-Hu-96Tests 96 Tests
EUR 692

Human B-Cell Activating Factor (BAFF) ELISA Kit

DLR-BAFF-Hu-48T 48T
EUR 498
Description: A sandwich quantitative ELISA assay kit for detection of Human B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human B-Cell Activating Factor (BAFF) ELISA Kit

DLR-BAFF-Hu-96T 96T
EUR 647
Description: A sandwich quantitative ELISA assay kit for detection of Human B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human B-Cell Activating Factor (BAFF) ELISA Kit

RDR-BAFF-Hu-48Tests 48 Tests
EUR 522

Human B-Cell Activating Factor (BAFF) ELISA Kit

RDR-BAFF-Hu-96Tests 96 Tests
EUR 724

BIO

GL6265-1MG 1 mg
EUR 126

BIO

GL6265-25MG 25 mg
EUR 447

BIO

GL6265-5MG 5 mg
EUR 195

BIO

1673-1
EUR 158

BAFF (B-cell Activating Factor)

RC214-15 5ug
EUR 104.38

Mouse B-Cell Activating Factor (BAFF) ELISA Kit

RD-BAFF-Mu-48Tests 48 Tests
EUR 511

Mouse B-Cell Activating Factor (BAFF) ELISA Kit

RD-BAFF-Mu-96Tests 96 Tests
EUR 709

Rat B-Cell Activating Factor (BAFF) ELISA Kit

RD-BAFF-Ra-48Tests 48 Tests
EUR 534

Rat B-Cell Activating Factor (BAFF) ELISA Kit

RD-BAFF-Ra-96Tests 96 Tests
EUR 742

Mouse B-Cell Activating Factor (BAFF) ELISA Kit

DLR-BAFF-Mu-48T 48T
EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Mouse B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse B-Cell Activating Factor (BAFF) ELISA Kit

DLR-BAFF-Mu-96T 96T
EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Mouse B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat B-Cell Activating Factor (BAFF) ELISA Kit

DLR-BAFF-Ra-48T 48T
EUR 528
Description: A sandwich quantitative ELISA assay kit for detection of Rat B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Rat B-Cell Activating Factor (BAFF) ELISA Kit

DLR-BAFF-Ra-96T 96T
EUR 690
Description: A sandwich quantitative ELISA assay kit for detection of Rat B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Mouse B-Cell Activating Factor (BAFF) ELISA Kit

RDR-BAFF-Mu-48Tests 48 Tests
EUR 534

Mouse B-Cell Activating Factor (BAFF) ELISA Kit

RDR-BAFF-Mu-96Tests 96 Tests
EUR 742

Rat B-Cell Activating Factor (BAFF) ELISA Kit

RDR-BAFF-Ra-48Tests 48 Tests
EUR 558

Rat B-Cell Activating Factor (BAFF) ELISA Kit

RDR-BAFF-Ra-96Tests 96 Tests
EUR 776

Human B cell activation factorr from the tumor necrosis factor family receptor(BAFF-R)ELISA Kit

QY-E05097 96T
EUR 361

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